Journal of Plant Sciences
Volume 4, Issue 5, October 2016, Pages: 113-118

Growth and Ecophysiological Processes in Seedlings of Dipteryx odorata (Aubl.) Willd Submitted to Two Water Conditions

Bruno Moitinho Maltarolo1, 2, Ellen Gleyce da Silva Lima1, 2, *, Vitor Resende do Nascimento2, 3, Kerolém Prícila Sousa Cardoso1, 2, Tamires Borges de Oliveira4, Karollyne Renata Souza Silva2, 5, Juscelino Gonçalves Palheta1, 2, Liliane Corrêa Machado2, 5, Thays Corrêa Costa2, 5, Josilene do Carmo Mescouto de Sousa1, 2, Cândido Ferreira de Oliveira Neto1, 2, Roberto Cezar Lobo da Costa2

1Post graduate program in forest Science, Federal Rural University of Amazon, Belém, Brazil

2Biodiversity Study in Higher Plants, Federal Rural University of Amazon, Belém, Brazil

3Forestry Engineering, Federal Rural University of Amazon, Belém, Brazil

4Universidade Federal Rural da Amazônia, Belém, Brazil

5Agronomy, Federal Rural University of Amazon, Belém, Brazil

Email address:

(B. M. Maltarolo)
(E. G. da S. Lima)
(V. R. do Nascimento)
(K. P. S. Cardoso)
(T. B. de Oliveira)
(K. R. S. Silva)
(J. G. Palheta)
(L. C. Machado)
(T. C. Costa)
(J. do C. M. de Sousa)
(C. F. de O. Neto)
(R. C. L. da Costa)

*Corresponding author

To cite this article:

Bruno Moitinho Maltarolo, Ellen Gleyce da Silva Lima, Vitor Resende do Nascimento, Kerolém Prícila Sousa Cardoso, Tamires Borges de Oliveira, Karollyne Renata Souza Silva, Juscelino Gonçalves Palheta, Liliane Corrêa Machado, Thays Corrêa Costa, Josilene do Carmo Mescouto de Sousa, Cândido Ferreira de Oliveira Neto, Roberto Cezar Lobo da Costa. Growth and Ecophysiological Processes in Seedlings of Dipteryx odorata (Aubl.) Willd Submitted to Two Water Conditions. Journal of Plant Sciences. Vol. 4, No. 5, 2016, pp. 113-118. doi: 10.11648/j.jps.20160405.14

Received: August 3, 2016; Accepted: August 13, 2016; Published: September 12, 2016

Abstract: Cumaru is a tree originally from the Amazon. The objective was to analyze the parameters of gas exchange, relative water content, growth and biomass production in leaves and roots of Cumaru plants (Dipteryx odorata) submitted to drought. The experiment was conducted in a greenhouse at the Federal Rural University of Amazonia (UFRA), Belem-Para. The experimental design was completely randomized in split plot in time (four times evaluation and two water conditions: control and drought), with 5 repetitions, totaling 40 experimental units. Were applied Analysis of variance, standard deviations, statistical analyzes and the means were compared by Tukey test at 5% significance. Drought caused reduced in diameter, leaf biomass, transpiration, RWC at 7th day of treatment and increased stomatal resistance. The cumaru was not effective in retaining water in the system. The stomatal closure mechanism secured decreased transpiration in the plant loses less water, entertaining this mechanism also affect plant growth.

Keywords: Biomass, Dipteryx odorata, Gas Exchange, Greenhouse, Split Plot in Time

1. Introduction

Cumaru (Dipteryx odorata) is a tree originally from the Amazon, its wood is appreciated in uses that require mechanical resistance to weathering and deterioration by termites and wood decay fungi. The seeds of this species contains the essential oil called cumarina and the exploration of this oil in the chemical industry has the potential to enable non-timber extraction [1]. The cumaru is a kind of widely dispersed throughout the Amazon, being common in floristic surveys of the region [2], and found in places with small to moderate water deficiency in Amazonas, Acre, Pará, Rondônia and northern of Mato Grosso [3]. There is great concern with the current forecasts indicate that global warming and increased drought in many regions of the planet [4]. And with that, as water resources become scarce, the commercial exploitation of plants tolerant to drought becomes a priority to obtain high yields [5]. Making it necessary to choose species with genotypes tolerant to drought, ensuring income to farmers environments with water scarcity or drought periods. In this sense, the understanding of plant responses to drought is of fundamental importance for understanding the variation of crop water consumption in its different stages of development, being able to interfere with the physiological aspects involved in the process, as well as on their consequences [6].

The studies related to stress conditions are important for physiological ecology and are in tools that enable the evaluation of the limits of tolerance and survival, as well as the adaptation of these species to the conditions of natural stresses [7]. The water deficit is one of the main limiting factors of development and growth of plants, only the species with drought resistance mechanisms can thrive in environments with water limitation [8].

Dry periods may increase the development of the roots, and accumulating assimilates them, which increases the soil water absorbing layer which has water available [9]. Cell growth appears as the most sensitive response to the lack of water in the soil, a small reduction in soil water potential causes decrease in cell growth [10]. The reduction or complete cessation of growth is considered first and the most serious consequence of physiological water deficit for plants, since it compromises cell elongation [11]. To [12] this fact is a standstill in vegetative growth occurring direction of assimilates produced to the reproductive organs.

In this way, it is essential the ecophysiological study of this species in its early stages of growth, mainly by assisting in the establishment of indicative physiological parameters of tolerance to abiotic stresses. Parameters such that if well used and managed, can improve the production and establishment of new seedlings, and to facilitate the selection of more resistant genetic material desired climate conditions [13].

The parameters of gas exchange, growth and biomass, can help in the understanding of the physiological processes of cumaru under drought conditions. The aim of this study was to analyze the gas exchange parameters, relative water content, growth and biomass production in leaves and roots of cumaru plants (Dipteryx odorata) submitted to drought.

2. Material and Methods

2.1. Local and Experimental Conduction

The experimente was conducted in a greenhouse of Federal Rural University of Amazonia (UFRA), belonging of Agrarian Sciences Institut (ICA), located in Belém-Pará, in a period of three months in 2015. The seedlings of cumaru (Dipteryx odorata (Aubl.) Willd.), from seeds that were provided throught AIMEX (Associação das Indústrias Exportadoras de Madeira do Estado do Pará) with four months old, were placed in plastic pots with a capacity of 3.6 L. The substrate consisted of yellow dystrophic Latosol. Before the start of treatment, all plants were irrigated daily for three months, corresponding to the acclimation time to keep them at field capacity.

Was added 5 ml of solution containing macro and micronutrients in all samples at the start of acclimation in the form of nutrient solution [14], modified in the lab of Biodiversity studies in higher plants (EBPS), UFRA.

The plants were subjected to two water regimes: irrigated (control) and water drought, in which the imposition of drought was obtained by suspension of irrigation in a period of 21 days.

2.2. Experimental Design and Statistics

The experimental design was completely randomized in split plot in time (four times evaluation and two water conditions: control and drought), with 5 repetitions, totaling 40 experimental units, each experimental unit was composed of a plant / pot. It was applied to analysis of variance in the results and when there was a significant difference, the means were compared by Tukey test at 5% significance level. Moreover, the standard deviations for each treatment were calculated, and statistical analysis performed by the program ASSISTAT Versão 7.7.

3. Relative Water Content (RWC)

The RWC was determined at 06:00 h in each collection. The method utilized was the described by [15]. The results were expressed as a percentage, according to the formula below: RWC = (FM1 – DM)/(FM2 – DM) x 100 (%)

Em que:

FM1= Fresh Mass 1;

FM2= Fresh Mass 2 (with saturation);

DM= Dry Mass;

3.1. Gas Exchange

The stomatal conductance and transpiration (E) were determined by a portable dynamic balance porometer (mod. 1600 Li, liquor, Nebraska, USA).

Measurements were performed 9:00 am under natural light with photosynthetically active radiation (PAR) around 680 µE m−2 s−1, and relative humidity (RH) of 80% and air temperature (Tair) 30°C. As samples, mature leaflets were selected and fully expanded from the second or third pair leaves counted from the apex.

3.2. Growth E Biomass

The height of the proposed plant was the total height corresponding to the length that goes from stem base to the apex for such measurement a graduated ruler was used in centimeters. The stem diameter was measured with a digital caliper.

Biomass production was measured in the act of each destructive sampling (at 0, 7, 14 and 21 days of water deficiency) where the plants were separated into root, stem and leaf. This biomass after drying in forced circulation oven at 70°C for 72 hours was used to determine the dry mass of root, stem dry weight, dry weight of leaf and total dry mass in analytical balance.

4. Results

The Relativa Water Content (RWC) present in the leaves of tonka bean under water stress decreased with the passing of days and average the plants controls had water percentage between 87.7% and 85.5% while the plants under deficiency between 88.3% and 37.5%. The significant reduction of RWC was noted from 7 days of water suspension, reaching very low values after the 21 days of stress as shown in Figure 1.

Figure 1. Relative Water Content in Young plants of Cumaru submitted to drought. Capital letters show statistical differences between water conditions and lower statistical differences between the collection times were compared by Tukey test at 5% probability.

Figure 2. Root Biomass (A), Stem (B), Sheet (C) and total (D) in young plants of Cumaru subjected to drought. Capital letters show statistical differences between water conditions and lower statistical differences between the collection times were compared by Tukey test at 5% probability.

The biomass od the root showed values between 4.25 and 5.97 g in plants and controls 4.25 g and 5 g in drought (Figure 2A). In stem the values were 2.8 to 4.7 g (control) and 2.58 to 4.05 g (drought) (Figure 2B). The leaves was 4.88 to 5.97 g (control) and from 4.9 to 3.99 g (drought) (Figure 2C). The total biomass was 11.93 to 16.6 g (control) and from 11.73 to 13.04 g (drought) as shown in Figure 2D.

The results of the high end of the experiment had no significant difference, the values were 37 cm for the control plants and 35 cm under drought for plants (Figure 3A). The stem diameter showed a statistically significant difference with values of 6.89 cm in plants controls and 5.75 cm in plants under drought (Figure 3B).

Figure 3. Plant height (A) and Diameter (B) of the Stem in young plant Cumaru subjected to drought. Capital letters show statistical differences between water conditions and lower statistical differences between the collection times were compared by Tukey test at 5% probability.

The transpiration dropped significantly in plants under drought throughout the experiment and the stomatal resistance had the opposite behavior (figure 4A). Initially the transpiration was of 4,22 mmol.m-2.s-1 and to 21th day was of 1,24 mmol.m-2.s-1 in plants under drought. The stomatal resistance in time 0 was of 5,14 and in time 3 of 18,2 (figure 4B).

Figure 4. Transpiration (A) and Stomatal Resistance (B) in young plants of Cumaru subjected to water deficit. Capital letters show statistical differences between water conditions and lower statistical differences between the collection times were compared by Tukey test at 5% probability.

5. Discussion

The RWC on the sheets is correlated with the water availability in the soil as well as the efficiency of the plant to assimilate water in adverse conditions and maintaining water in the plant reducing- losses.

The photosynthesis is a process that promotes water loss through the stomata and the low availability of water in the soil decreases the TRA [16].

[17] working with Carica papaya L found a RWC of 59% after 20 days of drought, results came to the RWC of Cumaru with 14 days under drought (figure 1).

The biomass of root and stem of tonka bean presented a growth trend over time in both water conditions, but in both parties this increase was not statistically different (Figure 2). The biomass of leaves showed different behavior of root and stem, in which there was a drop to the 21 day of the experiment in plants under drought. The total biomass reflects the sum of the effects occurred in the biomass of the other part of the plant it was noticed a statistical difference between the control plants and drought at 21 days of stress, it is probably due for the loss of biomass by senescence, and unfortunately these leaves that fell was not possible to account.

Among the morphological characteristics, the leaf area is the first affected by drought due to decreased leaf growth, possibly with senescence as a way of reducing transpiration area [18].

[19] states that the plants under dry treatments tend to reduce the growth of aerial parts in order to promote root growth in search of water. However, the species studied in this study did not present this strategy.

In a selection of seedlings it is necessary to evaluate various parameters accordingly height is a very important variable to be easy to measure and not destructive. The height of the plants studied in this study showed little difference between the treatments and there is no statistical difference between the collection times and enter not into the water conditions apply. The non height growth of Cumaru plants is because of this kind have very slow growth. This statement may be given to analyze the height of the control plants he found in adequate conditions of development and yet not grown in height, then the height of the Cumaru seedlings did not differ between treatments by intrinsic characteristic behavior of this species.

The diameter was probably affected by the decrease of the cell elongation by turgor force that consists of promoted positive pressure by water in tissuem cells called extension tissue mechanism [20].

With the increased in stomatal resistance is expected to decrease transpiration, as well as the fall of stomatal conductance that are inversely correlated. According to [21] decreased stomatal conductance is related to decreased water potential. In this present study the water potential was not measured, but it seems the lack of water in the soil water potential changes Cumaru also decreased triggering the locking mechanism of the stomata. The stomatal closure prevents transpiration and therefore water loss [22].

The Cumaru was not effective in retaining water in the system to present marked reduction in relative water content when subjected to water suspension.

Among the variables height, biomass stem and root did not respond to the stress period keeping average of 36 cm, 4.8g and 3.5g respectively.

Dought caused reduced growth in diameter, leaf biomass, transpiration and increased stomatal resistance.

The stomatal closure mechanism secured decreased transpiration in the plant loses less water, however this mechanism also affect plant growth.


  1. Lorenzi, H. Árvores Brasileiras: Manual de Identificação e cultivos de plantas arbóreas do Brasil. 2ª Ed. São Paulo: Nova Odessa. 2002.
  2. Embrapa, Considerações sobre o manejo da estrutura diamétrica do cumarú-ferro (Dipteryx odorata), em empresa no estado do Acre. Colombo- PR, p. 298, julho, 2012.
  3. Carvalho, P. E. R. Cumaru-Ferro Dipteryx odorata. Comunicado técnico Colombo- PR, p. 225, julho, 2009.
  4. Carmo, M. S. do; Borges, L. P.; Torres Junior, H. D.; Santos, P. G. de F.; Matos, F. S. efeito da Disponibilidade de Nitrogênio e Déficit Hídrico no Crescimento Inicial de Plantas de Pinhão Manso. Revista Agrotecnologia, Anápolis, v. 5, n. 2, p. 33 - 48, 2014.
  5. M atos, F. S.; R osa, V. R.; B orges, L. F. O.; R ibeiro, R. P.; C ruvinel, C. K. L.; D ias, L. A. S. Response of Jatropha curcas Plants to Changes in the Availability of Nitrogen and Phosphorus in Oxissol. Journal of Academic Journals, Lagos, v. 9, p. 3581-3586, 2014.
  6. Peixoto, C. P.; Cerqueira, E. C.; Soares Filho, W. S.; Castro Neto, M. T.; Ledo, C. A. S.; Matos, F. S.; Oliveira, J. G. Análise de crescimento de diferentes genótipos de citros cultivados sob déficit hídrico. Revista Brasileira de Fruticultura, Jaboticabal, v. 28, n. 3, p. 439-442, 2006.
  7. Guedes, R. S. et al. germinação e vigor de sementes de Apeiba tibourbou submetidas ao estresse hídrico e diferentes temperaturas. Ciência Florestal, v. 23, n. 1, p. 45-53, 2013.
  8. González, R. M.; Ricardi, M. M.; Iusem, N. D. Epigenetic marks in na adaptive water stress-responsive gene in tomato roots under normal and drought conditions, Epigenetics 8: 864-872, 2013.
  9. Calvache, A. M.; Reichardt, K; Malavolta, E.; Bachi, O. O. S. Efeito da deficiência hídrica e da adubação nitrogenada na produtividade e na eficiência do uso de água em uma cultura do feijão. Scientia Agricola, 54: 232-40, 1997.
  10. Salisbury, F. B.; Ross, C. W. Fisiologia das plantas. 4ª Edição, Cengage Learning, 858 p., 2012.
  11. Larcher, W. Ecofisiologia vegetal. Tradução: Prado, C. H. B. A. São Carlos: Ed. Rima, 2006, p. 531.
  12. Larcher, W. Ecofisiologia vegetal. São Carlos: Rima Artes e Textos, 2000. 531p.
  13. Nogueira, R. J. M. C.; Silva, E. C. Comportamento estomático em plantas jovens de Schinopsis brasiliensis Engl. Cultivadas sob estresse hídrico. Iheringia, Ser. Boat., Porto Alegre, v. 57. N. 1, p. 31-38, junho 2002.
  14. Hoagland, D. R. e Arnon, D. I. The water culture method for growing plants without soil. Calif. Agric. Csp. Stn. Univ. Calif. Berkeley Cir. p. 137: 147, 1950.
  15. Slavick, B. Methods of studyng plant water relations. Springer Verlang, p. 449, 1979.
  16. Lobato, A. K. S. et al. Physiological and biochemical behavior in soybean (Glycine max cv. Sambaiba) plants under water deficit. Australian Journal of Crop Science, Austrália, v. 2, n. 1, p. 25-32, 2008.
  17. Silva, R. T. L.; Oliveira Neto, C. F.; Barbosa, R. R. N.; Costa, R. C. L.; Conceição, H. E. O. Respostas fisiológicas de plantas de mamoeiro submetido ao déficit hídrico. Nucleus Animalium (Online), v. 9, p. 1-9, 2012.
  18. Mar, C. da C.; Conceição, H. E. O. da; Santos, A. B. R. dos; Viégas, I de J. M.; Silva, F. S. N. da; produção de massa seca e área foliar do açaizeiro sob déficit hídrico. Revista Agroecossistemas, v. 5, n. 2, p. 14-23, 2013.
  19. Díaz-Lopez, L.; Gimeno, V.; Simón, I.; Martínez, V.; Rodríguez-Ortega, W. M.; García-Sánchez, F. Jatropha curcas seedlings show a water conservation strategy under drought conditions based on decreasing leaf growth and stomatal conductance. Agricutural Water Management, v. 105, p. 48-56, 2012.
  20. Kerbauy G. B. Plant Physiology. Guanabara Koogan S. A., Rio de Janeiro, 2004.
  21. Nogueira, G. A. dos S. Respostas ecofisiológicas e bioquímicas de plantas jovens de Ochroma pyramidale (Cav. Ex Lam) Urb. Submetidas à deficiência hídrica e alagamento. Dissertação (Mestrado em Ciências Florestais) – Universidade Federal Rural da Amazônia, 2015.
  22. Alves, G. A. R.; Santos, F. B. G. dos.; Lobato A. K. da S.; Yuen T. D. K; Oliveira Neto, C. F. de; Costa, R. C. L. da, Ávila, F. W.; Marques, D. J; Galate, R. S. dos. Water relations, nitrogen compounds and enzyme activities in leaf and root of young Yellow Lapacho (Tabebuia serratifolia) plants subjected to flooding. Plant Omics Journal, v. 5, n. 3, p. 216-222, 2012.

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