Detection of Most Pathogenic Bacteria in Renal Failure and Urinary Tract Infections Patients with Antibiotics Patterns

Urinary tract infection (UTIs) are some of the most common infections experienced by humans and most common cause of both community and nosoncomial infection for patients admitted to hospitals in worldwide. To shed light on this subject, present study was done to investigate relationship between bacterial infection and renal failure after treatment by follow up general urine examination, blood urea and serum creatinine. Two hundred and thirty samples has been taken from individuals their ages between (2 to 70) years old males and females these included one hundred and five urine samples from females and one hundred and twenty five urine samples from males. Samples has been tested by general urine examination by microscope and by chemical tested by (Strip urine-uorscan) by using number of chemical factors like (S. siliva celicielic acid, Benedict, Barium chloride Bacl, Ehrlich's, Foshus), also culture and antibiotics sensitivity test was done. Results of general urine examination appears two hundred and twenty two suffer from urinary tract infection percent 96.5% and 8 only with no urinary tract infection in 3.45% percent from urine samples. It has been taken two hundred and twenty two blood Samples from individuals who has urinary tract infection then detected (Blood urea, serum creatinine and urine sugar tests) to know relationship between bacterial infections and renal failure. Also in this study detected different types of bacteria pathogens in which isolated from patients with urinary tract infection with or without renal failure, Serotyping and antibiotics sensitivity test also done to known more suitable drug for each pathogens and antibiotic resistance.


Introduction
Urinary tract infection are some of the most common infections experienced by humans, exceeded in frequency among ambulatory patients by respiratory and gastrointestinal infections indeed bacterial infections of the urinary tract are the most common cause of both community acquired and nosoncomial infection for patient admitted to hospitals in the united states the prognosis and management at UTLS however depend on the site of infection and any predis posing factors [1].
Urinary tract infection are serious health problem affecting millions of people each year million in the united state alone and it was the second most common type of infection more frequently in women than in men furthermore approximately 20% of women who recurrence [2].
Urinary tract infection are relatively easy to diagnose A clean cache urine specimen is used and urine determine the presence of a high level of white blood cells which may indicate a bacteria may then be cultured to determine the type of bacteria and susceptibility test par antibiotic should be used to treat the infection.
However urinary tract infections also account for up to 40% of nasocomail infections catheters these hospitalacquired infections tend to be more serious because the bacteria resistant to drug treatment and patients are often in poor general health [3].
Urinary tract infection account for more than 7 million visits to physicians offices french epidemiologic study evaluated is annual incidence at 53000 diagnoses per million per year which represent to 1.05% to 2.10% of the activity of general practitioners in females was estimated to be 250000 The incidence of urinary tract infection is higher among females in who it commonly accurse in an anatomically normal urinary tract conversely in males and children [4].

Materials and Methods
The following instruments were used throughout the study Electric Oven Binder (Germany) 6.
Sensitive electronic balance Denver 8.
Burner (Japan)     All culture media were prepared according to information of manufacturing company. Table 7. Prepared media.

No.
Name of Medium 1.

Results
These study included (230) patients suffered from urinary tract infections with or without renal failure from Al-zahraa and Al-Karama teaching hospital in Wassit governorate in period from October 2009 to October 2010 in age between (2-70) years old in different gender, urine and blood samples was collected from them, after culturing of all urine samples on different media the results showed that 194 (84.3%) gave positive result compared with 36 (15.7%) negative, the blood samples used for detecting creatinine, urea and also fasting blood sugar was done to them, the results who were detected recorded in Table 8 below: In addition to that this study show low percentage from the results of (Jabir Alhashimy, 2005) that show infection 91% from patients with or without renal failure gave positive results.

Blood Urea and Serum Creatinine
Blood urea and serum creatinine tests was done to 222 patients in which suffered from urinary tract infections, the results showed 94 (42.3%) patients with different age and sex suffered from renal failure.
Results of this tests for E. coli bacteria positive for catalase, indole, methyl red and negative to oxidase, simmon citrate and vogas-postkawer test, also kelbsiella gave positive to catalase, siammon citrate, vogas-proskowres and negative to oxidase, indol and methyl red and also proteus gave the following result Table (9):   Table 9. Biochemical tests for different bacteria isolated from culture of human urine.

Sensitivity Test
The results of antibiotics sensitivity test to most common bacterial pathogens in which isolated in this study appeared in following table:

General Urine Examination (GUE)
After treatment the patients follow up by this test and also by sensitivity test using 25 antibiotic (

Blood Urea and Serum Creatinine
After treated of patient retest of blood urea and serum creatinine by take blood samples from the patients, the results of this testes showed the percentage of recovered patients from renal failure 45 (47.8%) from 94, ratio of treated renal failure at aged between (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)  Also noted that all patients suffered from renal failure had urinary tract infections but very low numbers had firstly urinary tract infections them infected with renal failure for this case appeared that renal failure enhanced ability to grow different bacteria them can lead to infected with urinary tract infections.

Conclusion
According to our study and results achieved we can conclude the following: 1. The result of this study shows found most 3 types of bacteria in which caused of urinary tract infection (E. Coli, Klebsiella and proteus). 2. The result of this study shows its high number at patient that age between (41-70) years old suffering from renal failure. 3. The result at this study shows it high number at patient that have suffering from renal failure that have suffering from urinary tract infection. 4. The result of this study shows it's the percent of covered to patient that have suffering from renal failure and urinary tract infection low from percent of covered to patient that had suffering from urinary tract infection only. 5. The results of this study shows it's all types of bacteria caused urinary tract infection sensitive to many antibiotic 6. The results of this study shows it's all type at bacteria caused urinary tract infection resistance to some antibiotics. 7. The result of this study shows its high number of individual that live in village and poor that had urinary tract infections more than percent the individual that live in center city and rich man. 8. The result of this study showed that E. Coli bacteria found in large number in age between 2-15 years old. 9. The results of this study showed high number of patient that suffering from diabetic metallic and urinary tract infections.